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• Dried blood spot controls included

• Multiple diagnoses in a single run

• High selectivity and sensitivity by MS/MS detection

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The Chromsystems reagent kit for the determination of amino acids and acylcarnitines from dried blood spot samples using tandem mass spectrometry is intended for the early diagnosis of amino acid and fatty acid metabolic disorders during newborn screening.

In Germany, newborn screening is regulated by law. Twelve diseases are defined, eight of them to be determined by tandem mass spectrometry. As therapies for only these specific diseases are known, the diagnosis of other diseases is prohibited. Included in these diseases are defects of amino acid metabolism (phenylketonuria and maple syrup urine disease), defects of fatty acid metabolism (Medium and Very Long Chain Acyl-CoA Dehydrogenase Deficiencies) and organoacidurias (isovaleric acidemia). If not detected, these metabolic errors may impair the newborn severely and irreversibly within the first days of its life.57000 MassChrom® Amino Acids and Acylcarnitines/Non Derivatised, Reagent kit for LC-MS/MS analysis

• All required and isotope labelled standards included

• Dried blood spot controls included

• Direct sample injection without derivatisation

• High selectivity and sensitivity by MS/MS detection

This Chromsystems reagent kit allows the very fast and reliable determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the filter card without any further sample preparation steps required. The test utilises stable isotope labelled internal standards for calibration and measurement to ensure reliable and precise quantification of the analytes. Due to the time saving sample preparation and high precision of the MRM method, this direct injection kit stands out from the derivatised method. On the other hａnd lower sensitivity and interferences from isobaric substances (e.g. C5DC and C6OH) in acylcarnitines analyses may occur.

This Chromsystems reagent kit allows the very fast and reliable determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the filter card without any further sample preparation steps required. The test utilises stable isotope labelled internal standards for calibration and measurement to ensure reliable and precise quantification of the analytes. Due to the time saving sample preparation and high precision of the MRM method, this direct injection kit stands out from the derivatised method. On the other hａnd lower sensitivity and interferences from isobaric substances (e.g. C5DC and C6OH) in acylcarnitines analyses may occur.